Specimens for cytogenetic studies cannot be stored for too long, as cells lose their viability. For the best results, all cultures should be set up as soon as possible to ensure maximum possibility of successful growth.
Lithium heparin blood can rarely be used 10 days after collection, as cells division ceases. Blood specimen may be stored in the fridge at 2 - 8°C.
EDTA blood specimens may be stored up to 24 hours at room temperature.
Long term storage should be in the fridge at 2 - 8°C.
Bone Marrow specimens, collected in either EDTA (lavender tube) for RT-PCR or Ph-chromosome analysis only or Heparine (green tube) for all the other analysis, must be transported to the laboratory at room temperature within 24 hours. Bone marrow may be held at room temperature without processing for up to 24 hours. Bone marrow for FISH / Chromosome analysis can be stored for 72 hours at 2 - 8°C.
Amniocentesis specimens should be cultured within 48 hrs of collection, as there is a rapid decrease in the culture success rate after this time. Amniotic fluid must be stored in the fridge at 2 - 8°C. Aliquots (1 ? 2 ml) of specimen are stored at -20oC as a backup for AFP-testing.
CVS specimens received in transport medium should be set up as promptly as possible after collection. Only if the specimen arrives at the laboratory very late at night, should it be stored either in the fridge at 2 - 8°C or in a 37°C incubator overnight.
Tissue culture specimens received in transport medium should be set up as promptly as possible after collection. In the case where same day delivery is not possible, the specimen should be stored either in the fridge at 2 - 8°C or in a 37°C incubator overnight.
Extracted genomic DNA specimens may be transported at room temperature.
Blood spots on Guthrie Cards can be stored in a dry area at room temperature for several years.